TALENs

TALENs: timeline of key events

Moscou, M J, Bogdanov, A J, 'A simple cipher governs DNA recognition by TAL effectors', Science, 326 (2009), 1501; Boch, J, Scholze, H, Schornack, Landgraf, A, Hahn, S, 'Breaking the code of DNA binding specificity of TAL-Type III effectors', Science, 326 (2009), 1509-12.2009-12-11T00:00:00+0000TALENs are restriction enzymes secreted by Xanthomonas bacteria when they infect plants. They can be engineered to cut out specific sequences of DNA. The genome editing method was published in M Christian, T Cermak, E L Doyle, et al, 'Targeting DNA double-strand breaks with TAL effector nucleases', Genetics, 186 (2010), 757-761.2010-07-26T00:00:00+0000Zhang, F, Cong, L, Lodato, Kosuri, S, Church, G, Arlotta, P, 'Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription', Nature Biotechnology, 29 (2011), 149–53. 2011-01-19T00:00:00+0000Osborn, M J, Starker, C G, McElroy, A N, 'TALEN-based gene correction for epidermyolysis bullosa', Molecular Therapy, 21/6 (2013), 1151-9.2013-06-01T00:00:00+0000The pigs, a small breed known as Bama, had some of their genes disabled. They were developed for use in studying stem cells, gut microbiota, and Laron syndome, a type of dwarfism caused by a mutation in the human GHR gene. The announcement was made at Shenzhen International Biotech Leaders Summit.2015-09-23T00:00:00+0000One year old Lalya Richards was given an experimental form of gene therapy using genetically engineered immune cells from a donor to help treat her acute lymphoblastic leukaemia which she had from when she was just three months old. The cells were edited using the TALENs genome editing technique by Paul Vehys and Waseem Qasim. Within a month the new cells had killed off all the cancerous cells in Layla's bone marrow. 2015-11-05T00:00:00+0000UK scientists show how the TALENs gene editing tool can be used to switch on the immune system to stop cancer. L. Menger, et al, 'TALEN-Mediated Inactivation of PD-1 in Tumor-Reactive Lymphocytes Promotes Intratumoral T-cell Persistence and Rejection of Established Tumors', Cancer Research, 2016, doi: 10.1158/0008-5472.CAN-15-3352. 2016-04-15T00:00:00+0000
Date Event People Places
11 Dec 2009Transcription activator-like effectors, proteins secreted by the plant pathogenic bacteria (Xanthomonas), are sequencedMoscou, Bogdanove, Boch, Scholze, Schornack, Landgraf, HahnIowa State University
26 Jul 2010Genome editing technique using transcription activator-like effector nucleases (TALENs) publishedChristian, Cermak, Doyle, Schmidt, Zhang, Hummel, Bogdanove, VoytasUniversity of Minnesota, Iowa State University
19 Jan 2011US scientists publish a technique for genetically engineering transcription activator-like effectors customised to recognise specific DNA-binding sites which they show is an effective tool for editing mammalian genomesZhang, Cong, Lodato, Kosuri, Church, ArlottaHarvard University, Massachusetts Institute of Technology
1 Jun 2013Basic studies conducted with TALENs to see if can correct mutant genes associated with Epidermolysis Bullosa, a rare inherited skin disorderOsborn, Starker, Colby, McElroyUniversity of Minnesota, National Centre for Tumor Diseases Heidelberg, German Cancer Research Centre, Harvard University
23 Sep 2015Beijing Genomics Institute announced the sale of the first micropigs created with the help of the TALENs gene-editing techniqueBeijing Genomics Institute
5 Nov 2015First successful use of gene therapy to treat baby dying from leukaemiaVehs, QuasimGreat Ormond Street
15 Apr 2016Gene editing used to prompt immune cells to combat cancerQuezada, Johnson, Menger, Sledzinska, Bergerhoff, Vargas, Smith, Poirot, Pule, Hererro, PeggsUniversity College London, Cancer Research UK, Cellectis

11 Dec 2009

Transcription activator-like effectors, proteins secreted by the plant pathogenic bacteria (Xanthomonas), are sequenced

26 Jul 2010

Genome editing technique using transcription activator-like effector nucleases (TALENs) published

19 Jan 2011

US scientists publish a technique for genetically engineering transcription activator-like effectors customised to recognise specific DNA-binding sites which they show is an effective tool for editing mammalian genomes

1 Jun 2013

Basic studies conducted with TALENs to see if can correct mutant genes associated with Epidermolysis Bullosa, a rare inherited skin disorder

23 Sep 2015

Beijing Genomics Institute announced the sale of the first micropigs created with the help of the TALENs gene-editing technique

5 Nov 2015

First successful use of gene therapy to treat baby dying from leukaemia

15 Apr 2016

Gene editing used to prompt immune cells to combat cancer