CRISPR-Cas9: Timeline of key events

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Nelson, C E, Hakim, C H, Ousterout, D G, Thakore, P I, et al, 'In vivo genome editing improves muscle function in a mouse model of Duchenne muscular dystrophy', Science, DOI: 10.1126/science.aad51432015-12-31T00:00:00+0000Kleinstiver, B P, Pattanayak, V, Prew, M S, Tsai, S Q, Nguyen, N T, Zheng, Z, Joung, K, 'High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects', Nature, doi:10.1038.2016-01-06T00:00:00+0000The UK Human Fertilisation and Embryology Authority give the go ahead to a team at the Crick institute, led by Kathy Nikan, to edit human embyos to undestand the genetic cause of miscarriage. 2016-02-01T00:00:00+0000A.C. Komor, Y.B. Kim, M.S. Packer, J.A. Zuris, D.R. Liu, 'Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage', Nature, 533/7603 (2016), 420–24.2016-05-16T00:00:00+0000Work led by Carl June at University of Pennsylvannia to treat patients with multiple myeloma, melanoma and sarcoma.2016-06-21T00:00:00+0000NAS and NAM, Human Genome Editing: Science, Ethics and Governance, February 20172017-02-24T00:00:00+0000J. S. Gootenberg, O.O. Abudayyeh, J. W. Lee, et al, 'Nucleic acid detection with CRISPR-Cas13a/C2c2', Science, 13 April 2017, eaam9321, DOI: 10.1126/science.aam9321 2017-04-13T00:00:00+0000The experiment was carried out by scientists at Sichuan University, Temple University and the University of Pittsburgh. It was published in C. Yin, et al, 'In Vivo Excision of HIV-1 Provirus by saCas9 and Multiplex Single-Guide RNAs in Animal Models', Molecular Therapy, 25/5 (2017), 1168-86.2017-05-13T00:00:00+0000M. Hong, N. Marti-Gutierrez, S-W Park, et al, 'Correction of a pathogenic gene mutation in human embryos', Nature, doi:10.1038/nature233052017-08-02T00:00:00+0000UK scientists modified 41 embryos shortly after fertilisation. N.M.E. Fogarty et al, 'Genome editing reveals a role for OCT4 in human embryogenesis', Nature, doi:10.1038/nature240332017-09-20T00:00:00+0000P. Liang, et al, 'Correction of beta-thalassemia mutant by base editor in human embryos', Protein and Cell (2017), doi.org/10.1007/s13238-017-0475-6.2017-09-23T00:00:00+0000
Date Event People Places Sciences
31 Dec 2015CRISPR successfully used to improve muscle function in mouse model of Duchenne muscular dystrophy, opening way to use CRISPR to correct genetic mutatiuons in affected tissues of sick patientsNelson, Gersbach, Hakim, Ousterout, ThakoreDuke University, University of Missouri, University of North Carolina, Massachusetts Institute of Technology, Harvard UniversityCRISPR-Cas9, Gene editing, Gene therapy
6 Jan 2016US scientists published improved version of CRISPR/Cas 9 with less risk of off-target DNA breaksKleinstiver, Pattanayak, Prew, Tsai, Nguyen, Zheng, JoungHarvard UniversityCRISPR-Cas9, Gene editing
1 Feb 2016UK scientists authorised to genetically modify human embryos using CRISPR-Cas 9NiakanCrick InstituteCRISPR-Cas9
16 May 2016US scientists publish new base editing technique offering means to alter genome without needing to cleave double-stranded DNA or for a donor DNA templateKomor, Kim, Packer, Zuris, LiuHarvard UniversityCRISPR-Cas9, Gene editing
21 Jun 2016US NIH Recombinant DNA Advisory Committee approved first study in 15 patients using CRISPR/Cas 9 to genetically modify immune cells to attack cancerJuneUniversity of PennsylvaniaCRISPR-Cas9, Gene editing, Gene Cancer immunotherapy
Feb 2017US National Academies of Science and Medicine issue report saying possible to proceed with CRISPR in germ-line experiments but urge caution  CRISPR-Cas9, Gene editing
13 Apr 2017CRISPR shown to be sensitive diagnostic tool for detecting single target of DNA or RNA moleculeAbudayyeh, Bhattacharyya, Collins, Daringe, Donghia, Dy, Essletzbichler, Freije, Hung, Joung, Koonin, Lee, Livny, Myhrvold, Regev, Sabeti, Gootenberg, Verdine, ZhangBroad Institute, Massachusetts Institute of Technology, Harvard University, Howard Hughes Medical Institute CRISPR-Cas9, Gene editing
13 May 2017Research published demonstrating how CRISPR-CAS9 can be used to eliminate HIV in infected mice. Yin, Zhang, Qu, Chang, Putatunda, Xiao, Li, Zhao, Dhai, Qin, Mo, Young, Khalili, HuTemple University, University of Pittsburgh, Sichuan UniversityCRISPR-Cas9, Gene editing
2 Aug 2017Research published demonstrating possibility of editing gene defect in pre-implanted human embryos for preventing inherited heart diseaseHong, Marti-Gutierrez, Park, Mitalipov, Kaul, Kim, Amato, BelmonteOregon Health & Science University, Salk Institute, Center for Genome Engineering, Seoul National University, China National GeneBank,CRISPR-Cas9, Reproduction, Cardiovascular, Gene editing
Sep 2017DNA of human embryos edited using CRISPR-Cas9 to study cause of infertilityFogarty, McCarthy, Snijders, Powell, Kubikova, Blakeley, Lea, Elder, Wamaitha, Kim, Maciulyte, Kleinjung, Kim, Wells, Vallier, Bertero, Turner, NiakanFrancis Crick Instiitute, Cambridge University, Oxford University, Seoul National UniversityCRISPR-Cas9, Gene editing, Reproduction
23 Sep 2017Chinese researchers report correction of gene linked to beta thalassaemia, inherited blood disorder, in human embryos using base editing techniqueLiang, Ching, Sun, Xie, Xu, Zhang, Xhiong, Ma, Liu, Wang, Fang, Songyang, Zhou, HuangSun Yat-sen University, Baylor College of MedicineCRISPR-Cas9, Gene editing, Reproduction

31 Dec 2015

CRISPR successfully used to improve muscle function in mouse model of Duchenne muscular dystrophy, opening way to use CRISPR to correct genetic mutatiuons in affected tissues of sick patients

6 Jan 2016

US scientists published improved version of CRISPR/Cas 9 with less risk of off-target DNA breaks

1 Feb 2016

UK scientists authorised to genetically modify human embryos using CRISPR-Cas 9

16 May 2016

US scientists publish new base editing technique offering means to alter genome without needing to cleave double-stranded DNA or for a donor DNA template

21 Jun 2016

US NIH Recombinant DNA Advisory Committee approved first study in 15 patients using CRISPR/Cas 9 to genetically modify immune cells to attack cancer

Feb 2017

US National Academies of Science and Medicine issue report saying possible to proceed with CRISPR in germ-line experiments but urge caution

13 Apr 2017

CRISPR shown to be sensitive diagnostic tool for detecting single target of DNA or RNA molecule

13 May 2017

Research published demonstrating how CRISPR-CAS9 can be used to eliminate HIV in infected mice.

2 Aug 2017

Research published demonstrating possibility of editing gene defect in pre-implanted human embryos for preventing inherited heart disease

Sep 2017

DNA of human embryos edited using CRISPR-Cas9 to study cause of infertility

23 Sep 2017

Chinese researchers report correction of gene linked to beta thalassaemia, inherited blood disorder, in human embryos using base editing technique